This Open Access volume provides comprehensive reviews and describes the latest techniques to
study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research
topic. Our knowledge about ribosome biogenesis and function such as transcription mRNA
modification and translation was the sine qua non for developing the powerful RNA-based
vaccines against RNA-viruses causing the world-threatening Covid-19 pandemia. The chapters in
this book are organized into six parts. Part One discusses a comparative survey about the unity
and diversity of ribosome biogenesis in pro- and eukaryotic cells. Part Two deals with the
genomic organization of eukaryotic rDNA and the role of RNA polymerase I in ribosomal RNA
transcription. Part Three explores in vitro methods to study RNA polymerase I structure and its
function and Part Four analyzes the nucleo-cytoplasmic transport of assembled ribosomes and
RNP complexes. Part Five covers modifications that increase the complexity of rRNAs and Part
Six provides readers with a review of eukaryotic translation and - for the first time -
describes a new method to analyze translation in vitro. Written in the highly successful
Methods in Molecular Biology series format chapters include introductions to their respective
topics lists of the necessary materials and reagents step-by-step readily reproducible
laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and
comprehensive Ribosome Biogenesis: Methods and Protocols is a valuable resource for scientists
and researchers interested in learning more about the increasing importance of in vitro
RNA-technologies.
