Mast Cells: Methods and Protocols Second Edition expands upon the previous edition with
current detailed reviews that cover topics of interest to mast cell neophytes and cognoscenti
alike. With new chapters on methods for the enumeration of tissue mast cells and isolation of
mature mast cells and mast cell progenitors from mammalian tissues Danio rerio (zebrafish)
model in the study of mast cell development and function the functions of mast cells in human
health and disease and methods for the isolation methods for the investigation of the
molecular mechanisms of mast cell activation and their effector functions assays for the
detection and analysis of mast cell secretory and cell surface phenotype and mast cell
activation state and experimental mouse models of disease that have been deemed useful for the
assessment of mast cell functions in the regulation of innate and adaptive immune response in
cancer tissue fibrosis auto-inflammation and allergic disease. Written in the highly
successful Methods in Molecular Biology series format chapters include introductions to their
respective topics lists of the necessary materials and reagents step-by-step readily
reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge Mast Cells: Methods and Protocols Second Edition provides a
sampler of useful methods and techniques in the further study of mast cell biology.
