This volume covers an array of techniques available for studying SH2 domains and
phosphotyrosine signaling. The book is divided into six parts: Part I outlines the history of
SH2 technology development and cell signaling Part II focuses on computational approaches
and tools used for identification classification and predictions of SH2 domain binding
partners Part III details various ways to prepare the SH2 domains as experimental reagents
Part IV presents methods for structural analysis and conventional binding assays using SH2
domains Part V describes high-throughput and proteomics approaches to aid in analyzing
SH2-mediated interactions and Part VI covers applications for SH2 domain to functional and
imaging analyzes. Written in the highly successful Methods in Molecular Biology series format
chapters include introductions to their respective topics lists of the necessary materials and
reagents step-by-step readily reproducible laboratory protocols and tips ontroubleshooting
and avoiding known pitfalls.Cutting-edge and thorough SH2 Domains: Methods and Protocols is a
valuable resource for computational biologists biochemists structural biologists cell
biologists pathologists and people interested in SH2 domains and phosphotyrosine signaling.
Researchers who are investigating how protein interaction domain mediate specificity in
signaling systems may also find this book informative.
