This detailed volume presents a set of protocols useful for researchers in the field of
recombinant immunoglobulin and alternative scaffold engineering aptamer development and
generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with
amino-acid based synthetic antibodies. Brief protocols about the generation of antibody
libraries are detailed as well as techniques for antibody selection characterization and
validation. This section is completed by a brief description of a bioinformatics platform that
supports antibody engineering during research and development. Part II contains basic
procedures about the selection and characterization of aptamer molecules and Part III
describes fundamental processes of MIP generation and application. Written for the highly
successful Methods in Molecular Biology series chapters include introductions to their
respective topics lists of the necessary materials and reagents step-by-step readily
reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical Synthetic Antibodies: Methods and Protocols is an ideal guide for
scientists seeking to propel the vital study of antibody research.
