Over the past decade there has been an increase in powerful proteomics technologies that allow
greater fundamental insights into the blood proteome. Further developments in informatic
analyses software developments and computational tools are providing insights into large data
sets open-source data along with large-scale application of bioinformatics. Serum Plasma
Proteomics: Methods and Protocols Second Edition is a comprehensive resource of protocols for
areas pre-analytical through to analytical of plasma and serum proteomics. Divided into five
convenient sections covering fractionation strategies for in-depth blood proteome analysis
defined procedures for blood collection handling and storage detailed protocols for
performing both antibody-based and non-antibody based quantitative assays proteome analysis of
blood cell compartments circulating nanomebraneous vesicles and blood-related fluids data
management statistical design and bioinformatic challenges. Written in the highly successful
Methods in Molecular Biology series format chapters include introductions to their respective
topics lists of the necessary materials and reagents step-by-step readily reproducible
laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative
and practical Serum Plasma Proteomics: Methods and Protocols Second Edition delivers a
valuable foundation for the development and application of blood-based proteomics and further
incorporates blood cell components including platelets red blood cells circulating
extracellular vesicles exosomes and related biofluids.
