This volume explores the latest methods used to study AMPK by computational biochemical
biophysical cellular and ex vivo and in vivo approaches. The chapters in this book cover
specific topics such as methods to measure change in cellular energy metabolism and analyze
metabolic pathways regulated by AMPK bioinformatics tools to identify AMPK targets knockdown
of AMPK by CRISPR-Cas9 production and crystallization of full-length human AMP-activated
protein kinase cell-free assays to measure the effects of regulatory ligands on AMPK use of
sensors of AMPK activity AMPK protein interaction by yeast two-hybrid the role of AMPK in
inflammation and autophagy analyzing the AMPK function in C. elegans and mammals (with special
focus on skeletal muscle blood vessels kidneys pancreatic islets and hypothalamus) and
human Gamma2 AMPK mutations. Written in the highly successful Methods in Molecular Biology
series format chapters include introductions to their respective topics lists of the
necessary materials and reagents step-by-step readily reproducible laboratory protocols and
tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough AMPK: Methods
and Protocols is a valuable resource for graduate students postdoctoral researchers and
established investigators who are interested in the richly diverse AMPK field.
