With the great cellular and therapeutic importance of plasma membrane proteins unbiased
technologies such as proteomics become even more vital as they have the power to define
patterns of membrane protein expression characteristic of distinct states of cellular
development differentiation or disease and thereby identify novel markers of or targets for
intervention in disease. In Membrane Proteomics: Methods and Protocols leading experts in the
field compile a laboratory bench resource which provides a comprehensive toolkit of proven
methods. The volume delves into various modifications to standard two-dimensional gel
electrophoresis protocols as well as liquid chromatographic methods protocols for the
enrichment of diverse classes of plasma membrane proteins in silico approaches and
cutting-edge techniques for quantitative membrane protein profiling. Written in the highly
successful Methods in Molecular Biology(TM) series format chapters include brief introductions
to their respective topics lists of the necessary materials and reagents step-by-step
readily reproducible laboratory protocols and notes on troubleshooting and avoiding known
pitfalls. Comprehensive and easy-to-use Membrane Proteomics: Methods and Protocols is an ideal
guide for investigators wishing to apply state of the art membrane proteomic methodologies in
their own research programs.
