Despite being known and studied for years peptides have never before attracted enough
attention to necessitate the invention of the term peptidomics in order to specify the study of
the complement of peptides from a cell organelle tissue or organism. In Peptidomics: Methods
and Protocols expert researchers present a comprehensive range of analytical techniques for
the analysis of the peptide contents of complex biological samples with an emphasis often on
higher throughput techniques suitable for the analysis of large numbers of peptides typically
present in the peptidomes. Encompassing a number of species ranging from bacteria to man the
methods presented intensively cover topics such as organism handling tissue and organ
dissection cellular and subcellular fractionation peptide extraction fractionation and
purification structural characterization molecular cloning and sequence analysis. Written in
the highly successful Methods in Molecular Biology(TM) series format chapters include
introductions to their respective topics lists of the necessary materials and reagents
step-by-step readily reproducible laboratory protocols and notes on troubleshooting and
avoiding known pitfalls. Comprehensive and cutting-edge Peptidomics: Methods and Protocols
brings this ten year old field fully up-to-date in order to inspire novices and experts alike
with the easy-to-follow practical advice needed to set up and carry out analysis of the peptide
contents of complex biological samples.
