This book focuses on innovative experimental and computational approaches for charting
interaction networks in bacterial species. The first part of the volume consists of nine
chapters focusing on biochemical and genetics and genomics approaches including yeast two
hybrid metagenomics affinity purification in combination with mass spectrometry
chromatin-immunoprecipitation coupled with sequencing large-scale synthetic genetic screens
and quantitative-based mass spectrometry strategies for mapping the bacterial physical
functional substrate and regulatory interaction networks needed for interpreting biological
networks inferring gene function enzyme discovery and identifying new drug targets. The
second part comprises five chapters covering the network of participants for protein folding
and complex enzyme maturation. It also covers the structural approaches required to understand
bacterial intramembrane proteolysis and the structure and function of bacterial proteins
involved in surface polysaccharides outer membrane and envelope assembly. This volume
concludes with a focus on computational and comparative genomics approaches especially
network-based methods for predicting physical or functional interactions and integrative
analytical approaches for generating more reliable information on bacterial gene function. This
book provides foundational knowledge in the understanding of prokaryotic systems biology by
illuminating how bacterial genes function within the framework of global cellular processes.
The book will enable the microbiology community to create substantive resources for addressing
many pending unanswered questions and facilitate the development of new technologies that can
be applied to other bacterial species lacking experimental data.
