This first edition volume demystifies the complex topic of flow cytometry by providing detailed
explanations and nearly 120 figures to help novice flow cytometry users learn and understand
the bedrock principles necessary to perform basic flow cytometry experiments correctly.The book
divides the topic of flow cytometry into easy to understand sections and covers topics such as
the physics behind flow cytometry flow cytometry lingo designing flow cytometry experiments
and choosing appropriate fluorochromes compensation sample preparation and controls and ways
to assess cellular function using a variety of flow cytometry assays. Written as a series of
chapters whose concepts sequentially build off one another using the list of materials
contained within each section along with the readily reproducible laboratory protocols and tips
on troubleshooting that are included readers should be able to reproduce the data figures
presented throughout the book on their way to mastering sound basic flow cytometry
techniques.Easy to understand and comprehensive Flow Cytometry Basics for the Non-Expert will
be a valuable resource to novice flow cytometry users as well as experts in other biomedical
research fields who need to familiarize themselves with a basic understanding of how to perform
flow cytometry and interpret flow cytometry data. This book is written for both scientists and
non-scientists in academia government biotechnology and medicine.
