Offers a comprehensive and practical guide to the field of enzymology The highly anticipated
expanded third edition of the classic book on practical enzymology includes new lab-tested
assays for more than 30 enzymes across all enzyme classes. This brings the total up to 150
assay protocols for more than 110 different enzymes. The book also contains a new section on
the determination of enzyme-bound metal ions and covers special cases such as multi-enzyme
complexes binding measurements and immunoassays. Practical Enzymology 3rd Edition is
composed of two main parts - a general introduction into working with enzymes and a practical
part including step-by-step protocols for assaying enzymes including methods for protein
determination and enzyme immobilization. The book also addresses spectroscopic methods
electrochemical methods radioactive labeling and other instrumental methods. The chapters part
of the experimental section systematically covers assays for oxidoreductases transferases
hydrolases lyases isomerases and ligases (synthetases). Later chapters provide in-depth
coverage of multienzyme complexes assays enzyme relevant parameters and enzyme immunoassays.
The book finishes with two sections that look at binding measurements and methods and enzymes
in technical applications including the methods and modes for immobilized enzymes. -Expanded
with 30 new assays -Includes a new section on metal ion determination -Contains 150
ready-to-use assay protocols for the determination of the 110 most important enzymes -Presents
every assay in a user-friendly format filled with sage advice on the precise amounts of
chemicals for the assay solutions the correct formulae for calculating enzyme activity and
more -Covers special cases such as multienzyme complexes binding measurements and
immunoassays With its proven concept of concise and ready-to-use laboratory protocols coupled
with the necessary background information for successful experimentation and interpretation of
the results Practical Enzymology 3rd Edition will be a life-long companion for every
enyzmologist.
