Proteins interacting with diverse ligands - proteins peptides or DNA - are the basic
principles underlying many biological processes such as antigen-antibody binding signal
transduction or receptor binding. The technique of oligopeptide synthesis on a cellulose
membrane and the subsequent binding assays allow the investigation of protein interactions. A
particular advantage of these peptide arrays (SPOT - technology) is the high number of
oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis
and the analysis of protein-protein protein-DNA interactions as well as epitope mapping are
presented in this manual. It is ideally suited not only for basic research laboratories but
also for diagnostic and therapeutic applications since many diseases are related to
dysfunctions in protein recognition and binding.
