Despite effective chemotherapy fascioliasis remains a major public health problem in
developing countries with at least 17 million active infections resulting in significant
morbidity late infection detection and rapid reinfection after treatment. Therefore
alternative control strategies are mandatory. Proteases myofibrillar proteins are found only
in invertebrates. In the present study adult fresh Fasciola gigantica (F. gigantica) worms
were homogenized an antigen was purified and used to raise rabbit polyclonal antibodies (pAb).
The purified pAb was then used in sandwich ELISA to detect Fasciola antigens in sera samples
from a total 135 cattle.
