This book comprehensively describes the transgenesis techniques and applied experimental
methods in ascidians including enthusiastically developed original devices in addition to
concrete examples of developmental biology studies. Ascidians have been one of the most
important model animals in developmental biology for studying molecular and cellular processes
underlying formation of the chordate body plan. Transgenic techniques such as microinjection
electropolation cis-element analysis and application and TALENs and CRISPR Cas9 have been
developed in ascidians for more than 20 years and now many applied methods some of which are
unique in ascidians have been accumulated. Those extensive technological innovations such as
cell isolation cell labeling germ-line transformation marker transgenic lines and the
experimental systems for studying notochord formation and nervous system are exceptional
particularly in marine invertebrates. This book is useful for ascidian researchers to quickly
access the techniques in which they are interested as well as to compare each technology to
become familiar with specialized tips and for biologists of other organisms to learn the
unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily
understandable descriptions of techniques the book will inspire ascidian specialists to
improve their techniques encourage anyone wanting to begin studying ascidians and enable
readers to immediately apply the techniques to the organisms they research.
