This second edition provides 21 new chapters on methods used in laboratories for investigating
the physiology and molecular genetics of the pathogen Clostridium difficile. Chapters detail
up-to -date experimental techniques for gene editing and transcriptional analysis which are
used to investigate the fundamental biology of the organism and its virulence factors.
Additional chapters describe development of potential new treatments including vaccines
bacteriophage and faecal transplantation. Written in the highly successful Methods in Molecular
Biology series format chapters include introductions to their respective topics lists of the
necessary materials and reagents step-by-step readily reproducible laboratory protocols and
tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge
Clostridium difficile: Methods and Protocols Second Edition provides a comprehensive catalogue
of molecular tools and techniques authored by the researchers who have developed them.
