EAN: 9781493964406

GENOTYPING GEBUNDEN

This volume explores a broad range of different genotyping techniques. Genotyping: Methods and
Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed
microsatellite analysis  High Resolution Melt analysis and TaqMan-based assays  in situ
analysis of variants in single RNA molecules  the MassARRAY system and Molecular Inversion
Probes  Pulsed Field Gel Electrophoresis  Paralogue Ratio Test  and Multiplex
Ligation-Dependent Probe Amplification  long-range PCR combined with PacBio sequencing 
Targeted Locus Amplification  Multilocus Sequence Typing and rapid SNP detection with
pyrosequencing  and genotyping-by-sequencing for plant analysis. Finally  the volume concludes
with a summary of pertinent points to describe genetic variation. Written in the highly
successful Methods in Molecular Biology series format  chapters include introductions to their
respective topics  lists of the necessary materials and reagents  step-by-step  readily
reproducible laboratory protocols  and tips on troubleshooting and avoiding known pitfalls.
Thorough and practical  Genotyping: Methods and Protocols is a valuable resource for anyone
interested in learning more about the diverse field of genotyping.

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