This book addresses cutting-edge techniques for researching transposon mutagenesis an approach
for identifying individual gene contributions to the phenotypic characteristics of a particular
microorganism. The volume begins with methods for specific microorganisms and include protocols
for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium a
microorganism considered beneficial to humans and animals. The final section addresses more
general protocols including plasmid transfer and bioinformatic tools as well as novel
applications of transposon methodologies such as transposon-aided capture of antibiotic
resistant plasmids. Written for the highly successful Methods in Molecular Biology series
chapters include introductions to their respective topics lists of the necessary materials and
reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting
and avoiding known pitfalls. Authoritative and practical Microbial Transposon Mutagenesis:
Protocols and Applications serves as a valuable reference for scientists seeking to apply
transposon mutagenesis to microbial genetic analyses and functionality.
