Studies of membrane transporters have had a great impact on our understanding of human diseases
and the design of effective drugs. In Membrane Transporters in Drug Discovery and Development:
Methods and Protocols expert researchers provide practical methodologies of the ongoing
research on membrane transporters considering applications of transporter technologies in drug
discovery and development. Chapters include new and useful fields and methodologies including
pharmacogenomics nutrigenomics systems biology bioinformatics nuclear magnetic resonance
(NMR) imaging and quantitative real-time-PCR. Transporter studies in drug discovery and
development for various diseases are discussed including neuropsychiatric disorders
cardiovascular diseases ophthalmic diseases cancer and diabetes. Composed in the highly
successful Methods in Molecular Biology(TM) series format each chapter contains a brief
introduction step-by-step methods a list of necessary materials and a Notes section which
shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and current Membrane
Transporters in Drug Discovery and Development: Methods and Protocols delivers a collection of
practical protocols that can be used immediately in the lab along with critical surveys of key
topics by leading researchers in the field. TOC:Membrane Transporters and Drug Development:
Relevance to Pharmacogenomics Nutrigenomics Epigenetics and Systems Biology.- Bioinformatics
for Transporter Pharmacogenomics and Systems Biology: Data Integration and Modeling with UML.-
Multi Drug Resistance: Phylogenetic Characterization of Superfamilies of Secondary Carriers
that Include Drug Exporters.- Targeting Drug Transporters - Combining in Silico and In Vitro
Approaches to Predict in Vivo.- Methods to Evaluate Transporter Activity in Cancer.- Analysis
of Expression of Drug Resistance-Linked ABC Transporters in Cancer Cells by Quantitative
RT-PCR.- Fluorescence Studies of Drug Binding and Translocation by Membrane Transporters.- A
Model for Transport Studies of the Blood-Brain Barrier.- Genetic Variants in the Vesicular
Monoamine Transporter 1 (VMAT1 SLC18A1) and Neuropsychiatric Disorders.- Equilibrium Binding
and Transport by Vesicular Acetylcholine Transporter.- ABC Transporters in Ophthalmic Disease.-
Imaging of Protein Translocation in situ in Skeletal Muscle of Living Mice.- Glucose
Transporters in Parasitic Protozoa.- NMR Studies of Membrane Proteins.- Site-Directed
Mutagenesis in the Study of Membrane Transporters.- Xenopus laevis Oocytes.- Measurement of
Intracellular pH.- Measurement of Plasma Membrane Calcium-Calmodulin Dependent ATPase (PMCA)
Activity.- Assessment of the Contribution of the Plasma Membrane Calcium ATPase PMCA Calcium
Transporter to Synapse Function Using Patch Clamp Electrophysiology and Fast Calcium Imaging.
