The proteome consists of a complex mixture of proteins each of which need to be folded
correctly in order to function for the health of the organism and many of these proteins
require molecular chaperones to reach the correct conformation and in some cases to remain in
a folded form. In Molecular Chaperones: Methods and Protocols expert researchers address a
wide variety of approaches to the study these mechanisms featuring the workings of heat shock
proteins and heat shock transcription factors in vitro and in vivo. Written in the highly
successful Methods in Molecular Biology(TM) series format chapters features introductions to
their respective topics lists of the necessary materials and reagents step-by-step readily
reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge Molecular Chaperones: Methods and Protocols serves as an ideal
guide for all scientists who wish to pursue this vital biological action and its impact on
human health and disease.
