EAN: 9783954893850

ASSOCIATION OF PLANT GROWTH PROMOTING MICROORGA...

It was emphasized to engineering abiotic stress tolerance in blackgram by overexpression of the
glyoxalase I gene isolated from Brassica juncea under a most widely used CaMV 35S promoter. We
have demonstrated for the first the applicability of the glyoxalase system in imparting abiotic
stress tolerance in a crop legume Vigna mungo. The transgenic lines were confirmed for gene
insertion using different molecular techniques. The expression pattern of the inserted gene s
confirmed the expression and activity of the gene in the all transgenic lines studied up to T2
generations. The in vitro raised plants are usually sensitive to hardening process as well as
transplantation. In case of blackgram  the survival rate during hardening and the
transplantation was ca. 65-70%. To improve the percentage survival of plants  plant growth
promoting Rhizobium and Arbuscular Mycorrhizal fungi were applied during the hardening and
transplantation. This resulted in overall survival rate of plants up to 90% in both the
transgenics as well as the untransformed control plants. Effect of Rhizobium and Mycorrhiza on
transgenic as well as the untransformed control plants was studied. We reported for the first
time that the transgenic nature of the plant does not affect association of the plant growth
promoting Rhizobacteria or Mycorrhiza. Alleviates transplantation shock of in vitro grown Vigna
mungo plants was also observed.

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