EAN: 9781493967575

SIGNAL TRANSDUCTION IMMUNOHISTOCHEMISTRY GEBUNDEN

This second edition volume provides detailed protocols that address the challenges of
signal-transduction IHC. This book delves into chapters that discuss the nature of signal
transduction phenomena and approaches to making phosphor-specific antibodies  as well as
numerous bona fide methods methods on digital imaging techniques  preservation of tissue
targets  multicolor detection  flow cytometry  lipophagy analysis  apoptosis  and the
combination of IHC with in situ hybridization. Written in the highly successful Methods in
Molecular Biology series format  chapters include introductions to their respective topics 
lists of the necessary materials and reagents  step-by-step  readily reproducible laboratory
protocols  and tips on troubleshooting and avoiding known pitfalls.Thorough and comprehensive 
Signal Transduction Immunohistochemistry: Methods and Protocols  Second Edition is a valuable
resource to both novices and experts in other fields of biomedical research who need advice on
IHC protocols to study signal transduction. This book will also be useful for researchers in
academia  government labs  and the biotech industry.

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