This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO)
cells for recombinant protein production and protocols for the characterization of CHO cells
using ¿omic approaches. This book also explores methods that discuss the genome editing tool
CRISPR Cas9 and the characterization of recombinant protein products such as glycosylation
and host cell protein analysis. Written in the highly successful Methods in Molecular Biology
series format chapters include introductions to their respective topics lists of the
necessary materials and reagents step-by-step readily reproducible laboratory protocols and
tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge
Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for
scientists and researchers who are interested in further studying cell production in CHO cells.
