This volume examines established methods and protocols to isolate and characterize
extracellular vesicles (EVs) and their composition among other techniques including
purification imaging biofluid-specific and cell-specific isolation and downstream genomic and
proteomic profiling. The international group of expert scientists who have contributed to this
collection provide a variety of different techniques related to the growing assortment of EV
applications given that at times using only one technique or two is insufficient to address
the question at hand. Written for the highly successful Methods in Molecular Biology series
chapters include introductions to their respective topics lists of the necessary materials and
reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting
and avoiding known pitfalls. Comprehensive and practical Extracellular Vesicles: Methods and
Protocols serves as an ideal guide for researchers seeking to expand our knowledge of EV
functions and applications.
